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Sr. Research Associate

San Diego Metro Area, CA

Posted: 09/21/2018 Job Type: Scientific Job Number: JN -092018-22618
Description:
Summary/Scope
We are seeking a highly motivated individual with experience in next generation sequencing (NGS) to join our newly established Sequencing Research Group (SRG) at San Diego. The successful candidate will primarily be responsible for the operation and routine maintenance of the NGS operations within SRG and be able to support ongoing technology development needs as they arise. The ideal candidate should have a Master degree in a life science field with at least 2 years of direct experience in NGS operations or a Bachelor s degree with 4 or more years of direct NGS experience. Must be able to work independently as well as in a team environment. Be well organized with attention to details and can also multitask. Has the ability to learn quickly and is comfortable adapting to a new platform or new priority. Prior experiences in basic molecular biology procedures, such as DNA/RNA extraction and quantification, NGS library preparation, lab automation, strong computer skills and familiarity to LIM systems desired. Strong communication and computer skills required.

Responsibilities:
Perform routine operation and daily/weekly maintenance for sequencers and robotic systems as needed.
Assist SRG members with house-keeping tasks, such as lab space organization, freezer/refrigerator cleanup and organization, purchasing and organization of supplies, etc.
Extract and purify DNA and RNA from cells and tissues.
Quantitate and verify quality of DNA & RNA.
Prepare routine NGS libraries from bulk and single-cell DNA & RNA samples.
Maintain an accurate and complete laboratory notebook and other records as needed.
Enter data into company databases and data storage files.
Write and edit protocols and standard operating procedures.
Perform a wide variety of basic molecular biology techniques.

Skills and Knowledge Required:
Solid hands-on experience with basic molecular biology techniques such as:
DNA and RNA extraction and quantification.
Nucleic acid QC by chip and capillary electrophoresis.
PCR and qPCR.
Hands-on experience with NGS library preparation.
Hands-on experience in NGS sequencing using Illumina NextSeq500 and HiSeq4000.
Fluency in the use of Microsoft Word, Excel and PowerPoint.
Interest and proven aptitude in learning and applying new laboratory techniques, skills and instrumentation.
Great work ethics and self-motivated to seeing projects through successful completion.
Recognize unexpected outcome/problems; able to trouble-shoot as well as communicates and works with supervisor or technical support to solve the problem when needed.
Attention to detail and ability to perform delicate manual tasks in a laboratory.
Strong communication skills able to clearly communicate problems, protocols, and results to colleagues, both in writing and verbally and ability to keep complete and detailed records.
Able to interact with colleagues of diverse backgrounds and contribute to team goals.
Clear understanding of the general purpose and the goals of projects and how they fit into the organizational objectives.
Commit to quality by rigorous and constant evaluation of own work.
Demonstrates adaptability and being flexible; able to accept changes at any last moment s notice.
Ability to use technical skills and good time management to perform work efficiently.

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