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Senior Associate Scientist

San Diego Metro Area, CA

Posted: 10/31/2018 Job Type: Scientific Job Number: JN -102018-22946

The Structural and Chemical Biology department has an exciting opportunity for a Senior Associate Scientist in cell signaling and protein degradation. We are seeking an experienced and self-motivated individual to help our team design and analyze the activity of a library of engineered proteins using biochemical and/or cell-based strategies.

Responsibilities will include, but are not limited to, the following:
1. IMPORTANT: Development and implementation of biochemical assays using purified proteins and extension of these assays to high-throughput formats.
2. IMPORTANT: Development and implementation of cell-based assays to assess protein/protein interactions and the activity of signaling pathways.
3. Analysis of structure and activity relationships in engineered proteins.
4. Recombinant protein expression and purification from bacteria.
5. Clearly communicate results/data at project team meetings.
6. Maintaining an accurate and well-organized scientific notebook.

Skills/Knowledge Required:
-Optimization and analysis of complex cell-based and/or biochemical and enzyme assays.
-Experience with methods to interrogate protein/protein interactions in vitro, including HTRF and AlphaLISA, is highly desireable. Knowledge of other high-throughput screening technologies a plus.
-Construct design and protein expression in bacteria.
-Knowledge of protein purification strategies (i.e. affinity, ion-exchange, SEC) and the use of AKTA purifiers.
-Common methods for the analysis of purified proteins including SDS-PAGE and western blotting.
-Preparation of human tissue culture extracts and their analysis using western blotting and other standard molecular techniques. Experience with human tissue culture a plus.
-Experience interrogating small molecule-protein interactions.

Bachelor s degree in a scientific discipline with at least 8 years work experience or Master s degree with at least 6 years work experience.
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