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Scientist

San Francisco Metro Area, CA

Posted: 03/20/2019 Job Type: Scientific Job Number: JN -032019-23981

Job Description

The Translational Scientist will contribute to developing strategy and will be responsible for design and execution of lab experiments for translational studies supporting drug programs in late clinical development for hematological malignancies, including acute myeloid leukemia, myelofibrosis and myeloid dysplastic syndrome.

The ideal candidate will have translational and/or research experience in hematology and/or hematology-oncology, significant hands-on expertise in processing fresh blood and bone marrow samples, phenotypic and functional analysis of hematopoietic stem cells (HSCs) and blood. Other essential skills include multicolor flow cytometry, isolation anAddd culture of HSCs and blood cell fractions, co-culture models, and colony forming unit (CFU) assays.

Responsibilities will include, but are not limited to, the following:
o Process and analyze hematopoietic tissue samples and cell lines, including primary human and murine blood, bone marrow, and lymphoid tissues, utilizing FACS, MACS, lenti/retroviral transduction and varied assays to understand the biology of hematopoietic cells and the effect of drugs.
o Develop biochemistry and blood cell-based assays to understand the effect of therapeutic drugs, as single agents and in combinations using primary hematopoietic cells, including stem cells and blood.
o Develop ex-vivo cultures and outsource in vivo models to understand molecular and cellular aspects of hematological diseases, including blood cancers, drug pharmacokinetics, pharmacodynamics and drug mechanisms of efficacy to inform clinical biomarkers to guide selection of drug dose-schedule.
o Interact with project teams, academic collaborators and CROs, and critically review data generated by contract labs and academic collaborators.
o Present summaries of work at group meetings, internal forums and scientific conferences.
o Author study reports, assay protocols, patent applications, peer-reviewed publications.
o Contribute to develop strategy, design, execute and interpret lab experiments to characterize drug mechanisms of action and efficacy, understand mechanisms of resistance, and inform rational combinations for hematological malignancies.

Skills/Knowledge Required:
o B.Sc. or M.Sc (Scientist I) Ph.D. (Scientist II) degree in hematology, immunology, biochemistry, molecular or cellular biology, with at least 4 years of recent research or R&D experience in hematological diseases as a bench scientist or postdoc.
o Hands-on experience with phenotypic and functional assays used in hematology/hematology-oncology with understanding of cellular, biochemical and molecular readouts: these include analysis of primary samples (e.g. blood, bone marrow, immune cells) by multi-color flow cytometry; transduction and differentiation of hematopoietic stem cells (HSC) and immune cells; colony forming unit assays (CFU) and immune cell functional assays, and establishment of primary co-culture systems.
o Hands-on experience with assays to evaluate markers, signaling pathways, drug cytotoxicity, proliferation/apoptosis, multiplexed gene, cytokine, kinase profiling; ELISA/MSD, Western blot, RT-PCR, CRISPR/Cas9/RNAi.
o Comprehensive knowledge of ex vivo and in vivo models for hematology and immuno-oncology research, including transplantation, GEMMs, isogenic lines, and disseminated syngeneic and xenogeneic/xenograft models of hematological diseases. Handling of animals will not be required.
o Ability to develop a research plan, conduct experimental work with accurate and detailed record keeping.
o Excellent organizational skills, with the ability to efficiently manage and prioritize multiple projects, internal and external collaborations.
o Ability to interpret and summarize scientific data in an accurate, critical and concise manner.
o The candidate will be a critical experimentalist, results oriented, who enjoys working in the lab and is comfortable working in a fast-paced environment.
o Excellent oral and written communication skills.
o Strong independent problem-solving skills.
o Flexible and adaptable to changing priorities.
o Must be willing to work primarily in the lab.
o Title commensurate with educational background and experience.

Prerequisites:
o B.Sc. or M.Sc (Scientist I), Ph.D. (Scientist II) degree related to hematology, immunology, biochemistry, molecular or cellular biology, with at least 4 years of recent and demonstrable research or R&D experience in HSC biology or hematopoiesis or hematological diseases as a bench scientist are required. Research or R&D experience in hematology-oncology, myelogenous leukemia and/or myelofibrosis is a plus.

Essential skills: Hands-on experience in advanced multicolor (+6) flow cytometry, isolation and culture of HSCs and blood cell fractions, feeder-free and feeder-based culture models, and colony forming unit (CFU) assays.

o Candidates from Academic labs engaged in hematology or hematology-oncology research who fulfill above requirements are highly encouraged to apply.
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