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San Diego Metro Area, CA

Posted: 10/19/2018 Job Type: Scientific Job Number: JN -102018-22854
Position Objective

This scientist will execute service-based projects that primarily consist of genome engineering tool generation using the cutting edge cloning and genomic engineering techniques. This scientist will be expected to support projects and lead projects by leading cloning, sequencing, virus generation, and bio-informatics with an extensive use of automated liquid handling and laboratory information management systems. This scientist will act in a highly collaborative manner with the rest of the team through design, sequencing, analysis and large-scale manipulations of projects. Projects may include, but not be limited to subcloning, plasmid preparations, in vitro transcription of RNA, lentiviral production; these projects may be driven by internal project design or by external collaborations.

Scope of Need
The successful individual will directly interact and project management to execute a large number of specific project workplans. The scientist will be responsible for executing portions of these plans to meet agreed upon deliverables and timelines. These activities will require a high level of independence as well as the ability to work in a highly cooperative team.
This role will involve the following tasks:
Liquid handling:
o Experience using liquid handling instrumentation for cloning, sequencing (Sanger and NGS), cell culture and cell-based assays, and molecular biology
Cell Culture of adherent and suspension cell types:
o Transfection, transduction, antibiotic-selection, clonal expansion
o Flow cytometry
Molecular Biology:
o Plasmid subcloning, DNA preparations, DNA sequencing, in vitro transcription of RNA, packaging of lentivirus, PCR, RT-PCR, TaqMan qPCR
Genomic Editing:
o TALN and CRISPR based editing, design and implementation
Bio-informatics and Information Management
o Experience using bio-informatic approaches and tools in applied situations
o Experience using, designing, maintaining or administering LIMS systems to manage laboratory execution
o Specifically, experience with Nautilus LIMS will be a significant plus

Capabilities and Competencies
This position requires the following capabilities and competencies:
Multi-tasking and maintaining clear records of work performed
Proven sterile tissue culture technique
Use of automation for liquid handling
Ability to:
o Observe deviations from the expected outcomes and trouble-shoot
o Succeed with a moderate level of supervision and guidance
Strong communication and teamwork orientation with demonstrable influence/interpersonal skills
This position will benefit from:
Proven experience:
o Sequencing DNA on multiple platforms
o Flow cytometry
o Bioinformatics tools for design and analysis
o Using LIMS to support laboratory execution

Candidates with a bachelor s degree will be considered.
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