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Scientist

Boston Metro Area, MA

Posted: 11/22/2019 Job Type: Scientific Job Number: JN -112019-26025

Job Description

Duties:

We are seeking a flow cytometry core technologist to join our hematology research team developing therapies for hemophilia, sickle cell disease, and other non-malignant hematological disorders. The successful candidate will be responsible for independently setting up a range of flow-based experiments that include routine flow cytometry, complex highly multi-parametric analyses, hematopoietic single cell sorting, and other specialized cell characterization methods. The applicant will be responsible for maintaining flow cytometry equipment and training users on the proper design of experiments, use of equipment and correct data analysis. The qualified candidate should have a desire to build on their existing knowledge of cell phenotyping, and a willingness to take on new challenges toward the goal of building a state-of-the-art flow cytometry lab.


Responsibilities:

Perform cell sorting and manage sort requests.

QC instruments on a daily basis and troubleshoot problems; build and change cytometer configurations as needed.

Develop and perform multi-color staining assays for primary human and mouse phenotyping experiments.

Characterize and qualify reagents to be used in analysis and sorting assays, including FACS antibody panels and titers.

Perform data analysis using FlowJo and FACSDiva applications when needed.

Help train other users on all aspects of flow cytometry from planning experiments, acquisition and analysis.

Interpret and summarize experimental data and report summaries in team meetings, as needed.

Experience working in or running a core facility is a plus.

Experience running high content microscopy platforms, including Perkin Elmer Operetta and EMD Millipore ImageStream, and associated data analysis is a plus.

Experience with or a willingness to be trained on the operation of hematology analyzers (Advia, Beckman Coulter) is a plus.

Well versed in a variety of cell-based methods with a strong understanding of tissue culture, cell biology, immunoassays, and general laboratory techniques.


Qualifications:

PhD degree with 5+ years flow cytometry experience in appropriate scientific area related to position. A degree in cell biology, immunology, molecular biology, microbiology, or biochemistry is preferred.

Have an understanding of flow cytometry fundamentals and experience with analyzers including: BD LSR II, Canto II and Guava EasyCyte HT.

Experience cell sorting on a BD FACSAria Fusion

Comfortable developing and running 6+ color FACS panels.

General ability working with Windows and MAC operating systems and computer hardware/devices.

Proficiency with the following software: Microsoft Office, FACSDiva, FlowJo, MACSQuantify, Forecyte and Graphpad Prism.


Education:

PhD OR BA plus minimum 6 years of flow cytometry experience in an academic or biopharma setting OR master s degree plus minimum 3 years of relevant research experience in an academic or biopharma setting.

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