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Research Scientist-Antibody Purification

San Diego Metro Area, CA

Posted: 09/06/2019 Job Type: Scientific Job Number: JN -092019-25559

Primary responsibility is to perform original research in order to develop marketable products.

Special emphasis will be placed on automated protein (antibody) purification, which will involve working with GE AKTA (Express / Pure) and Unicorn software.

Performs antibody and recombinant protein purification and analytical characterization with limited supervision.

o Protein Purification: concentrating supernatants, preparation and packing of chromatography columns, and conducting chromatography, including operating analytical and preparative fast performance and high-pressure chromatography systems.

o Analytical characterization: Endotoxin measurements, protein quantitation assays, analytical HPLC chromatography, and SEC analysis. Independently operates analytical instruments such as spectrophotometers, analytical HPLC, capillary electrophoresis, plate readers, light scattering systems, and biomolecular interactions analysis using Octet and BIAcore instruments.

o Formulation: formulation buffers preparation (molar concentration quantitation and pH adjustment) and formulation optimization

Develops new protocols and troubleshoots existing protocols.


KNOWLEDGE/SKILLS/ABILITIES (KSA's):


Bachelor's (B.S.) or Master s (M.S.) degree in biochemistry, organic chemistry, bioengineering, or equivalent; with 6+ of related experience and/or training.

REQUIRES **antibody purification** background (Minimum of 3 years).


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