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Molecular Biology Research Associate

San Diego Metro Area, CA

Posted: 06/15/2018 Job Type: Scientific Job Number: JN -062018-21850
  • Responsibilities will include, but are not limited to, the following:
  • BS / MS Molecular Biology or related with experience in molecular biology and plasmid cloning.
    Excellent mammalian cell culture technique.
    MUST HAVE: Fluorescent microscopy, qPCR, Westerns, cell proliferation assays, shRNA, CRISPR.
    Working knowledge of proteomic sample preparation & immunoprecipitation is desired.
    Experience using cancer cell models is desired.
  • Design and conduct in vitro cell biology experiments using mammalian cell lines.
  • Perform mechanism of action experiments with compounds and genetic manipulation.
  • Perform DNA/RNA/Protein analyses including qPCR, western blotting, immunoprecipitation, and mass spectrometry as necessary for each experiment.
  • Develop and optimize new assays for various projects.
  • Plasmid cloning: Gateway & Restriction cloning, Mutagenesis, PCR.
  • Help set up a proteomics sample preparation workflow and create new technologies.
  • Prompt notebook documentation. Present and publish findings.

  • Skills/Knowledge Required:
  • Highly experienced in molecular biology and plasmid cloning.
  • Excellent mammalian cell culture technique.
  • Fluorescent microscopy, qPCR, Westerns, cell proliferation assays, shRNA, CRISPR.
  • Working knowledge of proteomic sample preparation & immunoprecipitation is desired.
  • Experience using cancer cell models is desired.
  • Data analysis and data handling skills: Must be fluent using Windows PC & Excel. Knowledge of Python, R, Knime, or Vortex is a plus.
  • Previous industrial or academic lab experience in gene function studies.
  • Excellent organization and communication.
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