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Cell Biologist - Scientist

San Diego Metro Area, CA

Posted: 08/27/2018 Job Type: Scientific Job Number: JN -082018-22410
This scientist will execute service based projects that consist of high-throughput molecular biology and cell line generation activities that use cutting edge cloning and genomic engineering techniques from the Scientific portfolio. This scientist will be expected to lead some projects independently and to work with other scientists in a highly collaborative team environment. Projects may include, but not be limited to, cloning and DNA preparation, in vitro transcription of RNA, lentiviral production and cell line engineering, with cell engineering being the primary focus; these projects may be driven by internal project design or by external collaborations.
Scope of Need
The successful individual will directly interact with senior R&D scientists and project management to execute project workplans. The scientist will be responsible for executing or supporting these plans to meet agreed upon deliverables and timelines. These activities will require a high level of independence as well as the ability to work in a highly cooperative team.
This role will involve the following tasks:
  • Tissue culture (culture of many cell types)
  • Stable cell line generation (transfection, transduction, antibiotic-selection, clonal expansion)
  • Genomic editing (TALN and CRISPR based editing, design and implementation)
  • Molecular biology
    • Plasmid subcloning, DNA preparations, DNA sequencing, in vitro transcription of RNA, packaging of lentivirus, PCR, RT-PCR, TaqMan qPCR
    • Automation of plasmid cloning and molecular biology
This individual will also be expected to support and participate in R&D and project management meetings.
Capabilities and Competencies
This position requires the following capabilities and competencies:
  • Perform standard laboratory math calculations and solutions/media preparation
  • Proven sterile tissue culture technique
  • Proven experience manipulating DNA plasmid constructs and with various forms of PCR
  • Ability to observe deviations from the expected outcomes
  • Ability to succeed with a moderate level of supervision and guidance
  • Strong teamwork orientation with demonstrable influence/interpersonal skills
This position will benefit from:
  • Proven experience generating stable cell lines
  • Proven experience with genome engineering of mammalian cells
Associate s degree (required). Candidates with a bachelor s degree or industry experience (preferred
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