Cambridge Metro Area, MA
We are seeking a highly motivated Associate Scientist to join our RNA QC and annealing group and support RNAi-based research and drug discovery. The successful candidate will help in the quality control and annealing of oligonucleotides and siRNA s for research studies. Additionally, they will evaluate new formulation approaches for CNS and ocular delivery applications and develop suitable assays such as the Bacterial Endotoxins Test (BET) for improved quality control.
Summary of Key Responsibilities
Preparation of siRNA duplexes and QC of siRNA to support research and drug discovery.
Analysis and analytical method development of newly chemically modified oligonucleotides (single strands and siRNA duplexes).
Improve upon existing analytical methods and processes to expand the current capabilities.
Support siRNA formulation development for ocular and CNS applications.
Utilize analytical instrumentation such as UV-Vis spectroscopy, HPLC, and LC-MS for the analysis of both crude and purified synthetic products. Effectively aid in troubleshooting synthetic/process issues and independently find solutions.
Improve upon and/or develop new methods and automation for the downstream processing of synthetic oligonucleotides and their subsequent analysis/purification and annealing into siRNA duplexes.
Analyze, interpret, and organize analytical data to present to team members.
Experience with instrumentation such as HPLC, LC-MS, UV-Vis spectrometry, etc. is required.
Experience in developing new chromatographic methods (IEX/RP HPLC, SPE, LC-MS) is a plus.
Experience in solid-phase synthesis of biomolecules and a good understanding of the procedures involved in SPS are preferred.
Ability to function within a highly interdisciplinary environment is a must.
Excellent written and oral communication skills, good organization skills, the ability to multitask, and the ability to work within a team and/or independently are required.
Complete record-keeping and excellent laboratory documentation practice (electronic notebook) are required.
Strong commitment to high laboratory and scientific standards and the ability to maintain a safe, clean, and orderly laboratory work environment and assist in routine lab and equipment maintenance, vendor contract and requisitions.
Bachelor s or Master s degree in Chemistry, Biochemistry, or a related scientific field.
0-8 years of work experience, preferably in an industrial setting.