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Research Associate Protein Biochemistry

San Mateo Metro Area, CA

Post Date: 10/02/2017 Job ID: JN -102017-20134 Job Type: Biochemistry- Scientific
In this position, the successful candidate will apply her/his skills and expertise in protein biochemistry to assist in purifying protein targets bound by antibodies so that they may be identified using protein sequencing techniques. These methods include immunoprecipitation, chromatography and other protein biochemistry techniques.

  • Assist with antibody target identification efforts
  • Perform pilot and preparative immunoprecipitations on cell and tumor extracts
  • Purify proteins and other macromolecules using chromatographic methods
  • Generate lysates and subcellular fractions from tissue culture cells (including radiolabeled cells) and mouse tumors
  • Characterize purified material by 1D & 2D western blots, autoradiography
  • Work with other project team members and communicate results with the team
  • Follow laboratory safety guidelines and practices

  • Bachelor s degree in molecular biology, biochemistry or related discipline
  • REQUIRED: 1-6 years research experience performing protein purification and/or immunoprecipitation
  • Extensive experience with immunoblots, including slot and western blots
  • Experience with SDS-PAGE and electrophoretic transfer of proteins to membranes
  • Must have excellent trouble shooting, organization, and planning skills, and the ability to work under timeline in a fast-paced environment.
  • Excellent written and oral communication skills.

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